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Characterization of the cyp19a1a gene froma BAC sequence in half-smooth tongue sole (Cynoglossus semilaevis) and
作 者： SHAO Changwei, LIUGeng, LIU Shanshan, LIUChanglin, CHENSonglin 摘 要： The cyp19a1a gene encodes an aromatase that plays a key role in sex differentiation of the gonad. The first bacterial artificial chromosome (BAC) sequence of half-smooth tongue sole (Cynoglossus semilaevis) containing the intact cyp19a1a gene was reported and the conservation and synteny of the cyp19a1a gene among teleosts were analyzed in the study. The BAC is 107 367 bp in size, with an overall guanine-cytosine (GC) content of 43.44%, and contains 4.38% transposable elements. Nine genes were predicted, including seven functional genes and two hypothetical genes. The cyp19a1a gene of all tested teleosts has nine exons and eight introns, and potential binding sites flanking the transcriptional start site are conserved. The expression pattern among teleosts is also similar during ovarian differentiation. Synteny analysis revealed a conserved gene cluster PKH4B-SL9A5-FHOD3-CEBPG-CEBPA among teleosts. These findings suggest that, among teleosts, cyp19a1a genes not... -
Molecular cloning, subcelluar location and expression profile of signal transducer and activator of transcription 2 (STAT2) from turbot
作 者： Na Wang, Xian-Li Wang, Chang-Geng Yang, Song-Lin Chen 摘 要： Signal transducer and activator of transcription 2 (STAT2) is an important molecule involved in the type I interferon signalling pathway. To date, little STAT2 homologue is available in fish except Atlantic salmon and goldfish. In this paper, STAT2 was firstly cloned and characterized from turbot, a marine flatfish with high economic value. Briefly, turbot STAT2 cDNA is 3206 bp in length encoding a predicted protein of 793 amino acids. The phylogenetic tree shows that turbot STAT2 protein shared the closest relationship with Atlantic salmon. Analysis of subcellular distribution indicates that STAT2 is mainly present in the cytoplasm of TK cells -
cloning, genomic structure and expression analysis of ubc9 in the course of development in the half-smooth tongue sole
作 者： Qiaomu Hu, Songlin Chen 摘 要： The small ubiquitin-like modifier (SUMO) pathway is an essential biological process in eukaryote, and Ubc9 is an important E2 conjugating enzyme (UBE2) for SUMO pathway and plays a critical role in cellular differentiation, development and sex modification in various species. However, the relationship between Ubc9 and sex modification and development in fish remains elusive. To elucidate the impact of Ubc9 on sex modification and development, the full length of the cDNA and genomic sequence was cloned from half-smooth tongue sole, Cynoglossus semilaevis. Real-time quantitative RT-PCR demonstrated that ubc9 was ubiquitously expressed in different tissues, and the expression levels varied in the different stages of embryonic and gonadal development. In addition, the expression level was significantly higher in the temperature-treated females than the normal females and males. Moreover, the PET-32-Ubc9 plasmid was constructed... -
Iron-metabolic function and potential antibacterial role of Hepcidin and its correlated genes (Ferroportin 1 and Transferrin Receptor) in Turbot (Scophthalmus maximus)
作 者： Chang-Geng Yang, Shan-Shan Liu, Bing Sun, Xian-Li Wang, Na Wang, Song-Lin Chen 摘 要： Antimicrobial peptide plays an important role in fish immunity. The small molecular antimicrobial peptide Hepcidin in turbot was studied and reported in this paper. The Ferroportin 1 (FPN1) and Transferrin Receptor (TFR) genes, which are related to Hepcidin, were cloned in turbot. The characteristics of Hepcidin and its related genes were studied, including an analysis of the expression patterns and cloning of the Hepcidin promoter, the relationship between Hepcidin and NF-kB and the regulation of iron-metabolism. The results showed that the promoter of SmHepcidin contains the binding sites of NFkB, and NF-kB may directly or indirectly receive feedback signals from SmHepcidin. In the liver, spleen and kidney -
Polymorphism and Balancing Selection of MHC Class II DAB Gene in 7 Selective Flounder (Paralichthys olivaceus ) Families
作 者： Min Du, Song-lin Chen, You Liang, LeiWang,Feng-tao Gao, Yang Liu, and Xiao-Lin Liao 摘 要： In order to determine the genetic variation of the MHC class IIB exon2 allele in the offspring, 700 fry from seven families of Japanese flounder challenged with V. anguillarum were studied, and different mortality rates were found in those families. Five to ten surviving and dead fry from each of the seven families were selected to study the MHC class II B exon2 gene with PCR and a direct sequencing method. One hundred and sixteen different exon2 sequences were found and 116 different alleles were identified, while a minimum of four loci were revealed in the MHC class II B exon2 gene. The ratio (dN/dS) of nonsynonymous substitution (dN) to synonymous substitutions (dS) in the peptide-binding region (PBR) of the MHC class IIB gene was 6.234, which indicated that balancing selection is acting on the MHC class IIB genes. The MHC IIB alleles were thus being passed on to their progeny. Some alleles were significantlymore frequent in surviving than dead individuals... -
Construction of a High-Density Microsatellite Genetic Linkage Map and Mapping of Sexual and Growth-Related Traits in Half-Smooth Tongue Sole (Cynoglossus semilaevis)
作 者： Wentao Song, Yangzhen Li, Yongwei Zhao, Yang Liu, Yuze Niu, Renyi Pang, Guidong Miao, Xiaolin Liao, Changwei Shao, Fengtao Gao, Songlin Chen 摘 要： High-density genetic linkage maps of half-smooth tongue sole were developed with 1007 microsatellite markers, two SCAR markers and an F1 family containing 94. The female map was composed of 828 markers in 21 linkage groups, covering a total of 1447.3 cM, with an average interval 1.83 cM between markers. The male map consisted of 794 markers in 21 linkage groups, spanning 1497.5 cM, with an average interval of 1.96 cM. The female and male maps had 812 and 785 unique positions, respectively. The genome length of half-smooth tongue sole was estimated to be 1527.7 cM for the females and 1582.1 cM for the males. Based on estimations of the map lengths, the female and male maps covered 94.74 and 94.65% of the genome, respectively -
牙鲆遗传连锁图谱的构建
作 者： 庞仁谊, 宋文涛, 高峰涛, 廖小林, 赵永伟, 牛余泽, 王磊, 赵文，田永胜, 陈松林 摘 要： 利用基因组测序得到的大量微卫星序列, 以681383B 为父本、6812E36 为母本杂交获得的F1 为作图群体, 构建了牙鲆(Paralichthys olivaceus)微卫星标记(SSR)遗传连锁图谱。雌雄图谱共定位SSR 标记529 个, 其中雄性连锁图谱包括418 个标记, 分布在24 个连锁群上, 总长度1 418.1 cM, 标记平均间隔3.62 cM, 图谱覆盖率为88.7%; 雌性连锁图谱包括437 个标记, 分布在24 个连锁群上, 总长度1 298.1 cM, 标记平均间隔为3.16 cM, 图谱覆盖率为89.1%。牙鲆中密度遗传图谱的构建为QTL 分析以及分子标记辅助育种进一步奠定基础, 并可以有效推动牙鲆的遗传改良工作, 推动牙鲆养殖业的可持续发展。 -
Identification and expression analysis of goose type lysozyme in half smooth tongue sole (Cynoglossus semilaevis)
作 者： Zhen-Xia Sha, Qi-Long Wang, Yang Liu, Song-Lin Chen 摘 要： Lysozymes are considered to be potent innate immune molecules against the invasion of bacterial pathogens. The goose-type lysozyme is one of the three major distinct lysozyme types identified in the animal kingdom including teleosts. In this report, we identified, sequenced, and characterized the goosetype lysozyme gene (CsGLys) from half-smooth tongue sole (Cynoglossus semilaevis). The full-length cDNA of CsGLys is 1191 bp in length from the transcription start site to polyadenylation site, including a 91 bp 50-terminal untranslated region (UTR), a 452 bp 30-terminal UTR and a 648 bp open reading frame (ORF) of encoding a polypeptide with 215 amino acids. The deduced amino acid sequence of CsGLys possesses a Goose Egg White Lysozyme (GEWL) domain with three conserved residues (E91, D104 and D121) essential for catalytic activity. The CsGLys gene consisting of 2535 bp, was similar to those of other teleost species such as Japanese flounder and large yellow croaker with five exons... -
MHC polymorphism and disease-resistance to Edwardsiella tarda in six turbot (Scophthalmus maximus) families
作 者： DU Min, CHEN SongLin, LIU YanHong, NIU BaoZhen, YANG JingFeng & 摘 要： This study examined genetic variation in the major histocompatibility complex (MHC) Class II B gene in turbot (Scophthalmus maximus) by virulent bacterial pathogen challenge. One hundred fry from each of six families were infected with Edwardsiella tarda by intraperitoneal injection. Family mortality ranged from 28.0% to 83.3%. Complete exon 2 and intron 1 sequences of MHC Class II B genes were amplified from five survivor and five non-survivor individuals per family using the clone-sequence method. Thirty-seven sequences from 60 individuals revealed 37 different alleles, 25 of which were unique to this study. The 25 unique alleles belonged to 16 major allele types. Nine alleles were used to examine the association between alleles and resistance/susceptibility to disease. Five alleles were present in an individual, suggesting a minimum of three loci or copies of the turbot MHC Class II B gene. The rate of non-synonymous substitution (dN) was 2.30 and 1.58 times higher... -
牙鲆胚胎冷冻损伤的观察
作 者： 王春花 ，田永胜 摘 要： 为了观察牙鲆胚胎在冷冻保存过程中的形态受损情况，将其浸入20％PM(20％丙二醇和20％ 甲醇1：l的混合液)中平衡，并用程序化法和玻璃化法对其冷冻保存2h后解冻，用摄影显微镜记录其在抗冻剂里平衡时和冷冻保存后的形态。结果显示在平衡过程中胚胎卵膜出现凹陷(称为溶液损伤)，但可以恢复；在冷冻过程出现胞内冰损伤，是致命的；用程序化法冷冻保存的尾芽期胚胎卵膜和卵黄完好，胚体边缘受伤；用玻璃化法保存的尾芽期胚胎，卵膜和卵黄损伤较重，胚体损伤较轻；因此将玻璃化法和程序化法结合可以达到扬长避短的效果。 -
牙鲆胚胎程序化冷冻保存研究
作 者： 王春花，陈松林 ，田永胜 ，刘本伟 ，邓寒 摘 要： 以牙鲆胚胎为材料 ,对胚胎程序化冷冻保存的主要环节进行了探讨 ,以优化牙鲆胚胎的程序化超低温冷冻保存法。单一抗冻剂的毒性试验表明甲醇(Methanol) 、 1 ,22丙二醇( PG)的毒性低于其他抗冻剂的毒性。 -
牙鲆胚胎玻璃化冷冻技术研究
作 者： 田永胜，陈松林，严安生 摘 要： 对名优经济鱼类牙鲆 （Paralichthys Oliuaceus） 胚胎的玻璃化冷冻技术方法进行了研究。筛选出了适合于牙鲆胚胎平衡和培养的基础液 BS2， 对牙鲆不同时期胚胎在玻璃化液 VS2中的耐受能力进行了研究 -
牙鲆胚胎玻璃化冷冻及解冻过程中的温度因子与玻璃化率
作 者： 田永胜，刘本伟，陈松林 摘 要： 为避免鱼类胚胎玻璃化冷冻过程中脱玻璃化现象发生，本文对冷冻过程中温度变化及解冻方法进行了研究。在室温13℃下，将含有玻璃化液FVS3的麦管直接投入到一196℃ 的液氮中，麦管内温度达到一196℃ 时，所用的时间为15．46±1．38 s，降温速率为800。C／rain。对玻璃化麦管在35～45。C水浴中解冻时间进行测定。结果显示，解冻所需时间在6．17～7．94 S之间，麦管从一196℃升高到13。C的解冻速率为1 500~2 000。C／rain。当水浴温度从37℃逐步升高到72。C时，对水浴表面蒸气温度的变化进行了测定，相应水温下蒸气温度从25．47℃升高到38．6。C。在麦管中加入不同浓度的玻璃化液FVS1、FVS2、FVs3和FVS4进行玻璃化冷冻，然后将麦管分别在37℃ 水浴中和28．6℃的蒸气中解冻时玻璃化率进行研究。结果显示，在蒸气中解冻时的玻璃化率明显高于水浴中解冻；麦管玻璃化率在水浴中解冻时不稳定，在蒸气中解冻时表现稳定，玻璃化率随着玻璃化液浓度的升高有逐渐升高的趋势。对含有牙鲆胚胎的玻璃化冷冻麦管分别在37℃水浴和28．6℃蒸气中解冻，结果表明，蒸气中解冻时的玻璃化率同样明显高于水浴中解冻。 -
显微注射抗冻剂对牙鲆(Paralichthys olivaceus)胚胎毒性及冷敏感性的影响
作 者： 姜静，田永胜，翟介明，陈松林 摘 要： ：在鱼类胚胎冷冻保存中，由于胚胎体积大、卵膜具多层结构，并且通透性差，传统的平衡法无法使抗冻剂足够、有效的进入胚胎内部起到保护作用。为弥补了普通平衡法的不足，此研究利用显微注射技术，将抗冻剂直接注射到鱼类胚胎卵黄囊内，提高了抗冻剂对胚胎的保护程度。此研究以牙鲆胚胎为试验材料，对显微注射抗冻剂种类、抗冻剂的注射剂量、注射浓度、以及抗冻剂注射后胚胎的冷敏感性影响进行了筛选。结果表明：牙鲆胚胎较适宜的显微注射剂量为750pl；几种抗冻剂注射牙鲆胚胎后的毒性 大小相对排列为PVP>蔗糖，DMSO>MeOH>PG，其中注射PG获得的胚胎成活率和孵化率最高，注射PVP获得的胚胎成活率和孵化率最低，而PG与MeOH组成混合抗冻剂PM毒性更低获得的胚胎成活率和孵化率更高。而任何一种抗冻剂均随着浓度的增加其毒性随之增加；另外，在冷敏感试验中，显微 注射6 mol／L的PM的胚胎胚胎，应用程序化法处理，以2~C／min的速率由室温降至一20℃ ，平衡10 min后解冻处理，发现注射PM的胚胎低温处理后成活率为(25．07~1．57)％，而6mol／的PM五步平衡法同步处理的对照组胚胎成活率为(20．88士2．84)％ ，说明显微注射的胚胎冷敏感性一定降低。 -
鲈鱼胚胎的玻璃化冷冻保存
作 者： 田永胜， 陈松林，严安生，季相山，于过才 摘 要： 本文对鲈鱼 ( L ateolabrax japonicus) 胚胎进行了玻璃化冷冻保存研究 , 筛选出了浓度较低、玻璃化程度较稳定的5 种玻璃化液 , 冷冻时形成玻璃化的概率在4811 %～100 % , 在35～43 ℃ 的水浴中解冻时保持玻璃化的概率在 4414 %～6310 %; -
鲈鱼胚胎程序化冷冻保存的研究
作 者： 于过才 陈松林 孔晓瑜 田永胜 季相山 摘 要： 对鲈鱼 （Lateolabrax japonicus） 胚胎的程序降温冷冻保存进行了研究。通过稀释液的筛选实验， 选择了一种培养效果好的稀释液 DS1 液。 -
鲈鱼冷冻精子诱导半滑舌鳎胚胎发育
作 者： 田永胜 陈松林 邵长伟 刘本伟 庄志猛 摘 要： 半滑舌鳎是分布在我国黄、 渤海的特有鱼类 ,雌鱼明显具有较雄鱼生长快的特点 ,利用雌核发育方法诱导产生半滑舌鳎超雌鱼 ,通过与正常雄鱼交配 ,最终可培育生长速度快、 个体大的全雌性后代 ,对于提高养殖效益具有重要的意义。 -
花鲈冷冻精子诱导漠斑牙鲆雌核发育
作 者： 杨景峰， 陈松林， 徐亘博， 苏鹏志， 田永胜， 翟介明， 李 波 摘 要： 鱼类雌核发育工作开始于 20 世纪 50 年代，首先在淡水鱼类遗传育种的相关研究中迅速开展， 由于其母本遗传的特殊性， 雌核发育技术在基因作图与纯系的快速建立、 生产单性种群等方面有广泛的应用前景［ 1］ -
花鲈冷冻精液受精鱼苗与鲜精受精鱼苗的同工酶比较分析
作 者： 陈艳翠 ，高天翔 ，陈松林 ，季相山 ，田永胜 ，邓 寒 摘 要： 采用水平凝胶电泳技术对花鲈(Lateolabrax maculatus)冷冻精液受精鱼苗与鲜精受精鱼苗进行了同工酶分析。本实验选取肌肉和肝脏两种组织，检测了IDHP、PGM、LDH、GPI、MDH、G3PDH、ME、HK、SOD、G6PD和SDH等11种酶，得到13个位点：IDHP 、PGM一1 、PGM．2 、LDH 、GPI-1 、GPI-2 、MDH 、G3PDH 、ME 、HK 、SOD 、C．6PD 和SDH 。结果显示在0．99水平，SDH 和PGM一2 位点在两种受精鱼苗中均表现为多态，多态位点比例均为0．1538；平均预期杂合度分别为0．0460和0．0407，但未观测到杂合子，观测杂合度均为0，遗传偏离指数均为一1；有效等位基因数分别为1．0720和1．0850，花鲈冷冻精液受精鱼苗与鲜精受精鱼苗的遗传相似度为0．9991，遗传距离为0．0010 -
额尔齐斯河江鳕精子冷冻保存研究
作 者： 田永胜， 姜静， 马允， 向伟， 李胜忠， 马骁， 高坚， 范镇民， 钱龙 摘 要： 齐斯河中蕴藏着丰富的冷水鱼类种质资源，江鳕(厶施fo把)是其中之一，建立其精子冷冻保存技术和精子冷冻库，对种质资源保存具有重要意义。本研究利用无机盐、葡萄糖、蔗糖、胎牛血清等配制7种精子稀释液，以5：1的比例稀释精液，激活后观察统计精子活力。结果显示，江鳕精子仅在SS、D15和CM中具有(13．33±2．89)％～(8．33±2．88)％活 -
超低温冷冻保存对七带石斑鱼胚胎酶活性的影响
作 者： 姜静， 田永胜， 翟介明， 陈松林， JIANG Jing， TIAN Yong-Sheng， ZHAI Jie-Ming， CHEN Song- Lin 摘 要： 带石斑鱼(印i唧^ek sep￡e，咖sc泐凇)是一种生长速度快、低温下耐受性强具有较高经济价值的海水鱼类，在自然环境中种群数量较少，对其种质资源进行长期保存迫在眉睫。本研究以七带石斑鱼胚胎为实验材料进行种质冷冻保存，探讨超低温冷冻(一196℃)对七带石斑鱼胚胎内超氧化物歧化酶(superoxide dismutase，SOD)、肌酸激酶(creatine kinase，CK)、N。+瓜+．ATPase、乳酸脱氢酶(1actate dehydrogenase，LDH)、丙二醛(malondialdehyde，MDA)和谷胱甘肽过氧化物酶(glutathione peroxidase，GSH—PX)活性的影响，以检测抗冻剂在冷冻保存中对胚胎的影响。本研究分别测定了经过PM(24％1，2一丙二醇(PG)+16％甲醇(MeOH))、PMG(15．75％1，2一丙二醇+8．75％甲醇(MeOH)+8．75％甘油(Gly))和PMGT(15．75％1，2一丙二醇+8．75％甲醇(MeOH)+8．75％甘油(Glv)+5％海藻糖(trehalose))3种玻璃化液处理冷冻前后七带石斑鱼胚胎内6种酶活性的变化。结 -
Sperm cryopreservation of sex-reversed seven-band grouper, Epinephelus septemfasciatus
作 者： Seven-band grouper Epinephelus septemfasciatus is a protogynous hermaphrodite. The male individuals’ number is more less than the female one. Thus, it is necessary for artificial reproduction and crossbreeding to research the sperm cryopreservation of sex-reversed seven-band grouper. 摘 要： Seven-band grouper Epinephelus septemfasciatus is a protogynous hermaphrodite. The male individuals’ number is more less than the female one. Thus, it is necessary for artificial reproduction and crossbreeding to research the sperm cryopreservation of sex-reversed seven-band grouper. -
Sperm cryopreservation of sex-reversed seven-band grouper, Epinephelus septemfasciatus
作 者： Yongsheng Tiana,∗, Wenshan Qia,b, Jing Jianga,b, Na Wanga, Di Wanga,c, Jieming Zhaid, Chao Chena, Songlin Chena,∗ 摘 要： Seven-band grouper Epinephelus septemfasciatus is a protogynous hermaphrodite. The male individuals’ number is more less than the female one. Thus, it is necessary for artificial reproduction and crossbreeding to research the sperm cryopreservation of sex-reversed seven-band grouper. -
Long-term cryopreservation of sperm from Mandarin fish Siniperca chuatsi
作 者： Shuyan Dinga, Jiachun Gea,∗, Chen Haoa, Mingsheng Zhanga, Weihui Yana, ZhiqiangXua, Jianlin Pana, Songlin Chenb, Yongsheng Tianb, YahongHuang 摘 要： In order to develop cryopreservation techniques for long-termpre-serving the sperm of Mandarin fish Siniperca chuatsi, we examined the effects of various extender and cryopreservation on post-thawmotility. -
Effects of cryopreservation on the survival rate of the seven-band grouper (Epinephelus septemfasciatus) embryos
作 者： Yongsheng Tian a, *, 1, Jing Jiang a, b, 1, Lini Song a, b, Zhangfan Chen a, Jieming Zhai c, Jiangchun Liu c, Na Wang a, Songlin Chen a, * 摘 要： The effects of cryopreservation and the vitrification solution on the embryo hatchability of the seven- band grouper Epinephelus septemfasciatus were evaluated in this study. Six small molecule cryoprotec-tants (PG, MeOH, Gly, DMF, DMSO and EG) and four macromolecular cryoprotectants (glucose, fructose, sucrose and trehalose) were used to determine the embryo toxicity levels. -
Cryopreservation of sperm from turbot (Scophthalmus maximus) and application to large-scale fertilization
作 者： Song-Lin Chena,*, Xiang-Shan Jia,b, Guo-Cai Yua, Yong-Sheng Tiana, Zhen-Xia Shaa 摘 要： This paper reports on cryopreservation of sperm from turbot (Scophthalmus maximus). The effects of various extenders, cryoprotectants and sperm–egg insemination ratios on motility score and/or fertilization capacity of post-thaw turbot spermatozoa were examined to optimize cryopreservation procedures. -
Cryopreservation of spotted halibut (Verasper variegatus) sperm
作 者： Y.S. Tian a, S.L. Chen a,⁎, X.S. Ji b, J.M. Zhai c, L.J. Sun c, C. Chen a, P.Z. Su a 摘 要： Spotted halibut (Verasper variegatus) is now regarded as being endangered in China because of its continuous decline in the last three decades. Preservation and protection of its genetic resources were demanded in order to protect this rare fish species -
Cryopreservation of sea perch (Lateolabrax japonicus) spermatozoa and feasibility for production-scale fertilization
作 者： X.S. Jia, S.L. Chena,b,*, Y.S. Tiana, G.C. Yua, Z.X. Shaa, M.Y. Xua, S.C. Zhangc 摘 要： With the objective of germplasm conservation and cryobank construction, a method for cryopreserving sea perch (Lateolabrax japonicus, Cuvier) semen in 1.8-ml cryovials was developed. The effects of various extenders, cryoprotectants, volume of diluted semen on the motility score of post-thaw spermatozoa were examined. -
云纹石斑鱼心脏细胞系的建立与鉴定
作 者： 刘肖峰 陈松林 沙珍霞 吴娅红 于洋 王娜 修旺珊2 摘 要： 鱼类细胞系在鱼类病毒学及功能基因组学方面发挥着重要作用。云纹石斑鱼(Epinephelusmoara) 是一种具有重要养殖价值的海水鱼类， 尚未见细胞系建立的报道。本研究以云纹石斑鱼的心脏组织为材料， 建立了云纹石斑鱼心脏细胞系 -
半滑舌鳎脾脏"卵巢组织细胞离体培养的初步研究
作 者： 郑媛 陈松林 摘 要： 44半滑舌鳎#-$)*#1*33233,:"1&,F"3$ 属鲽形目#60>=?/C>23;Q/?9>A$ " 舌鳎科#-@C/E0/AA;B:>$ " 舌鳎属#-@C/E0/AA=A$ " 俗称牛舌头% 鳎目% 鳎米" 是一种暖温性近海大型底层鱼类"终年生活栖息在我国近海海区&1'"具广温%广盐和适应多变的环境条件的特点( 半滑舌鳎市场价值高"但自然资源量少"是我国新开发的重要海水养殖鱼类 -
Establishment and characterization of a gonad cell line from half-smooth tongue sole Cynoglossus semilaevis pseudomale
作 者： Ai Sun • Song-Lin Chen • Feng-Tao Gao • Hai-Long Li • Xiao-Feng Liu • Na Wang • Zhen-Xia Sha 摘 要： A new cell line was established from half-smooth tongue sole Cynoglossus semilaevis pseudoma-le gonad (CSPMG). Primary culture was initiated from gonad tissues pieces, and the CSPMG cells were cultured at 24 C in Dulbecco’s modified Eagle medi-um/F12 medium (1:1) (pH7.0), supplemented with 20 %fetal bovine serum, basic fibroblast growth factor -
Establishment and characterization of an ovarian cell line from half-smooth tongue sole Cynoglossus semilaevis
作 者： A. Sun, T. Z. Wang, N. Wang, X. F. Liu, Z. X. Sha and S. L. Chen 摘 要： A new ovarian cell line, CSO, was established from half-smooth tongue sole Cynoglossus semilaevis. Primary culture of CSO cells was nitiated from digestion of ovarian tissues pieces by trypsin solu-tion and cultured at 24∘C in Dulbecco’s modiied Eagle’s medium/F12 medium (DMEM/F12, 1:1) (pH 7⋅0), supplemented with 20% foetal bovine serum -
Establishment and characterization of a new fish cell line from head kidney of half-smooth tongue sole (Cynoglossus semilaevis)
作 者： Yuan Zheng • Na Wang • Ming-Shu Xie •Zhen-Xia Sha • Song-Lin Chen 摘 要： A new cell line (TSHKC) derived from half-smooth tongue sole (Cynoglossus semilaevis) head kidney was developed. The cell line was subcultured for 40 passages over a period of 360 days -
Establishment and Characterization of a Testicular Cell Line from the Half-Smooth Tongue Sole, Cynoglossus semilaevis
作 者： Bo Zhang1,2*, Xianli Wang1*, Zhenxia Sha1, Changgeng Yang1, Shanshan Liu1,2, Na Wang1, Song-Lin Chen1 Song-Lin Chen1 摘 要： Spermatogenesis within the adult testis is an excellent system for studying stem cell renewal and differentiation, which is under the control of testicular somatic cells. In order to under-standing spermatogenesis in the half-smooth tongue sole -
Establishment, characterization and virus susceptibility of a kidney-derived cell line from southern flounder, Paralichthys lethostigma Jordan & Gilbert
作 者： N Wang1 , X-L Wang1, Z-X Sha1, Y-S Tian1 and S-L Chen1 摘 要： Since the establishment of the first teleost fish cell line in the 1960s (Wolf 1962), more than 100 lines have been established and applied in many aspects of virology, immunology and genetics in freshwater and marine fish (Ferna ´ndez, Yoshimizu, Kimura, Ezura -
Development and characterization of a new marine fish cell line from turbot (Scophthalmus maximus)
作 者： N. Wang • X. L. Wang • Z. X. Sha • Y. S. Tian • S. L. Chen 摘 要： A new marine fish cell line, TK, derived from turbot (Scophthalmus maximus) kidney, was established by the method of trypsin digestion and subcultured for more than 50 passages over a period of 300 days. -
Establishment, characterization of a new cell line from heart of half smooth tongue sole (Cynoglossus semilaevis)
作 者： X. L. Wang • N. Wang • Z. X. Sha • S. L. Chen 摘 要： A new cell line was established from the heart of a culturedmarine fish, half smooth tongue sole (Cynoglossus semilaevis), designated as CSH (Cynog-lossus semilaevis heart cell line). -
Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis)
作 者： SHA Zhenxia1x, REN Guocheng1 2x, WANG Xianli 1 3x, WANG Na1, CHEN Songlin1¤ 摘 要： A new cell line, CSEC, has been successfully established from embryos at gastrula stage of a cultured marine ¯sh, half smooth tongue sole (Cynoglossus semilaevis). CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS (fetal bovine serum), -
Establishment and Characterization of a New Cell Line from the Kidney of Spotted Halibut Verasper variegate
作 者： WANG Xianli ， ，CHEN Songlin ’，SHA Zhenxia ，FAN Tingjun¨，and WANG Na ) 摘 要： In vitro culture of fish cells provides an important tool for studying cellular physiology,molecular biology,func—tional genomlcs，oxicology and transgenlc applications (Hightower and Renfro，1988；Bejar et a1． 2005；Bahich and Borenfreund，1 99 l：Gagn6 and Blaise，2000；Con．ception et a1．，2001：Oh et a1．，2001：Tiago and Laiz6．2008；Ma et a1．，200l：Pombinho et a1．，2004)． -
半滑舌鳎肝脏细胞系的建立与鉴定
作 者： 任国诚 陈松林 沙珍霞 摘 要： 以半滑舌鳎肝脏组织为材料，探索了组织细胞分离培养条件，建立了半滑舌鳎组织细胞培养技术及半滑舌鳎肝脏细胞系。该细胞系的培养基是添加了抗生素、胎牛血清、花鲈血清、成纤维生长因子的 。 形态呈纤维状，在培养基中生长迅速，经 多天的培养，成功传代30多代 -
漠斑牙鲆胚胎细胞系的建立与鉴定
作 者： 任国诚 陈松林 沙珍霞 摘 要： :漠斑牙鲆(Paralichthys lethostigma)是中国近年新开发的重要海水养殖鱼类, 本研究以漠斑牙鲆囊胚期胚胎为材料, 探索了鱼类胚胎细胞分离和培养的条件, 建立了漠斑牙鲆胚胎细胞培养技术。建立的漠斑牙鲆胚胎细胞系(SFEC)至今已经培养了240 多天, 传至80 多代。SFEC 的完全培养基采用添加抗生素、胎牛血清(FBS)、花鲈血清(SPS)、成纤维生长因子(bFGF)的DM EM 。SFEC 形态小而呈圆形、多边形, 在培养基中生长迅速。本实验检测了温度、胎牛血清浓度、成纤维生长因子对SFEC 生长的影响。在24 ～ 30 ℃之间SFEC 生长良好, 但当温度低于18 ℃时细胞生长速度明显减慢。在含15% FBS 的培养基中, SFEC 生长速度明显比在含7.5 % FBS 的培养基中快, 在培养基中添加bFGF 可以使细胞生长速度显著提高。SFEC 的二倍体核型为2n =6st42t 。GFP 报告基因通过脂质体介导的方法转入SFEC 中并成功地获得了表达, 转化率为20 %。[ 中国水产科学, 2007 , 14(4):579 -583] -
Pluripotency and Chimera Competence of an Embryonic Stem Cell Line from the Sea Perch (Lateolabrax japonicus)
作 者： Song-Lin Chen,1 Zhen-Xia Sha,1 Han-Qing Ye,1 Yang Liu,1 Yong-Sheng Tian,1 Yunhan Hong,2 Qi-Sheng Tang1 摘 要： A stable GFP-expressing (GFP+LJES1) cell strain was developed from the LJES1 cells obtained from sea perch (Lateolabrax japonicus,) embryos. GFP+LJES1 cells were induced in vitro by RA to differentiate into a variety of cell types and also had the ability to form embryoid body-like structures in suspension culture. To determine the differentiation potential of LJES1 cells in vivo, GFP+ LJES1 cells were trans-planted into sea perch and zebrafish embryos at mid-blastula stage. -
Development and characterization of cell lines from heart, liver, spleen and head kidney of sea perch Lateolabrax japonicus
作 者： H.-Q. YE*†, S.-L. CHEN*‡§, Z.-X. SHA* AND M.-Y. XU* 摘 要： Five cell lines (LJHK, LJS, LJL, LJH-1 and LJH-2) were established from the head kidney, spleen, liver and heart of sea perch Lateolabrax japonicus. The cell lines LJHK, LJS, LJL, LJH-1 and LJH-2 were subcultured 46, 32, 32, 36 and 34 times in minimum essential medium (MEM) supplemented with foetal bovine serum (FBS), sea perch serum and 10 ng ml 1 basic fibroblast growth factor (bFGF). -
Development and characterization of a continuous embryonic cell line from turbot (Scophthalmus maximus)
作 者： Song-Lin Chena,T, Guo-Cheng Rena,b, Zhen-Xia Shaa, Yunhan Hongc 摘 要： A continuous cell line, TEC (turbot embryonic cell line), was established from embryos at the gastrula stage of a cultured marine fish, turbot (Scophthalmus maximus), and has been cultured for more than 200 days with more than 60 passages. The TEC cells were cultured in DMEM medium supplemented with antibiotics, fetal bovine serum (FBS), sea perch serum (SPS), and basic fibroblast growth factor (bFGF). -
Establishment of a pluripotent embryonic cell line from sea perch (Lateolabrax japonicus) embryos
作 者： Song-Lin Chen *, Zhen-Xia Sha, Han-Qing Ye 摘 要： Pluripotent embryonic stem (ES) cells provide an efficient approach for genome manipulation with many applications in marine biotechnology and development studies. To develop this technology, we have worked to derive fish ES cells for in vitro studies of embryo cell growth and differentiation and for the generation of transgenic fish.