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Long-term cryopreservation of sperm from Mandarin fish Siniperca chuatsi
作 者： Shuyan Dinga, Jiachun Gea,∗, Chen Haoa, Mingsheng Zhanga, Weihui Yana, ZhiqiangXua, Jianlin Pana, Songlin Chenb, Yongsheng Tianb, YahongHuang 摘 要： In order to develop cryopreservation techniques for long-termpre-serving the sperm of Mandarin fish Siniperca chuatsi, we examined the effects of various extender and cryopreservation on post-thawmotility. -
Effects of cryopreservation on the survival rate of the seven-band grouper (Epinephelus septemfasciatus) embryos
作 者： Yongsheng Tian a, *, 1, Jing Jiang a, b, 1, Lini Song a, b, Zhangfan Chen a, Jieming Zhai c, Jiangchun Liu c, Na Wang a, Songlin Chen a, * 摘 要： The effects of cryopreservation and the vitrification solution on the embryo hatchability of the seven- band grouper Epinephelus septemfasciatus were evaluated in this study. Six small molecule cryoprotec-tants (PG, MeOH, Gly, DMF, DMSO and EG) and four macromolecular cryoprotectants (glucose, fructose, sucrose and trehalose) were used to determine the embryo toxicity levels. -
Cryopreservation of sperm from turbot (Scophthalmus maximus) and application to large-scale fertilization
作 者： Song-Lin Chena,*, Xiang-Shan Jia,b, Guo-Cai Yua, Yong-Sheng Tiana, Zhen-Xia Shaa 摘 要： This paper reports on cryopreservation of sperm from turbot (Scophthalmus maximus). The effects of various extenders, cryoprotectants and sperm–egg insemination ratios on motility score and/or fertilization capacity of post-thaw turbot spermatozoa were examined to optimize cryopreservation procedures. -
Cryopreservation of spotted halibut (Verasper variegatus) sperm
作 者： Y.S. Tian a, S.L. Chen a,⁎, X.S. Ji b, J.M. Zhai c, L.J. Sun c, C. Chen a, P.Z. Su a 摘 要： Spotted halibut (Verasper variegatus) is now regarded as being endangered in China because of its continuous decline in the last three decades. Preservation and protection of its genetic resources were demanded in order to protect this rare fish species -
Cryopreservation of sea perch (Lateolabrax japonicus) spermatozoa and feasibility for production-scale fertilization
作 者： X.S. Jia, S.L. Chena,b,*, Y.S. Tiana, G.C. Yua, Z.X. Shaa, M.Y. Xua, S.C. Zhangc 摘 要： With the objective of germplasm conservation and cryobank construction, a method for cryopreserving sea perch (Lateolabrax japonicus, Cuvier) semen in 1.8-ml cryovials was developed. The effects of various extenders, cryoprotectants, volume of diluted semen on the motility score of post-thaw spermatozoa were examined. -
云纹石斑鱼心脏细胞系的建立与鉴定
作 者： 刘肖峰 陈松林 沙珍霞 吴娅红 于洋 王娜 修旺珊2 摘 要： 鱼类细胞系在鱼类病毒学及功能基因组学方面发挥着重要作用。云纹石斑鱼(Epinephelusmoara) 是一种具有重要养殖价值的海水鱼类， 尚未见细胞系建立的报道。本研究以云纹石斑鱼的心脏组织为材料， 建立了云纹石斑鱼心脏细胞系 -
半滑舌鳎脾脏"卵巢组织细胞离体培养的初步研究
作 者： 郑媛 陈松林 摘 要： 44半滑舌鳎#-$)*#1*33233,:"1&,F"3$ 属鲽形目#60>=?/C>23;Q/?9>A$ " 舌鳎科#-@C/E0/AA;B:>$ " 舌鳎属#-@C/E0/AA=A$ " 俗称牛舌头% 鳎目% 鳎米" 是一种暖温性近海大型底层鱼类"终年生活栖息在我国近海海区&1'"具广温%广盐和适应多变的环境条件的特点( 半滑舌鳎市场价值高"但自然资源量少"是我国新开发的重要海水养殖鱼类 -
Establishment and characterization of a gonad cell line from half-smooth tongue sole Cynoglossus semilaevis pseudomale
作 者： Ai Sun • Song-Lin Chen • Feng-Tao Gao • Hai-Long Li • Xiao-Feng Liu • Na Wang • Zhen-Xia Sha 摘 要： A new cell line was established from half-smooth tongue sole Cynoglossus semilaevis pseudoma-le gonad (CSPMG). Primary culture was initiated from gonad tissues pieces, and the CSPMG cells were cultured at 24 C in Dulbecco’s modified Eagle medi-um/F12 medium (1:1) (pH7.0), supplemented with 20 %fetal bovine serum, basic fibroblast growth factor -
Establishment and characterization of an ovarian cell line from half-smooth tongue sole Cynoglossus semilaevis
作 者： A. Sun, T. Z. Wang, N. Wang, X. F. Liu, Z. X. Sha and S. L. Chen 摘 要： A new ovarian cell line, CSO, was established from half-smooth tongue sole Cynoglossus semilaevis. Primary culture of CSO cells was nitiated from digestion of ovarian tissues pieces by trypsin solu-tion and cultured at 24∘C in Dulbecco’s modiied Eagle’s medium/F12 medium (DMEM/F12, 1:1) (pH 7⋅0), supplemented with 20% foetal bovine serum -
Establishment and characterization of a new fish cell line from head kidney of half-smooth tongue sole (Cynoglossus semilaevis)
作 者： Yuan Zheng • Na Wang • Ming-Shu Xie •Zhen-Xia Sha • Song-Lin Chen 摘 要： A new cell line (TSHKC) derived from half-smooth tongue sole (Cynoglossus semilaevis) head kidney was developed. The cell line was subcultured for 40 passages over a period of 360 days -
Establishment and Characterization of a Testicular Cell Line from the Half-Smooth Tongue Sole, Cynoglossus semilaevis
作 者： Bo Zhang1,2*, Xianli Wang1*, Zhenxia Sha1, Changgeng Yang1, Shanshan Liu1,2, Na Wang1, Song-Lin Chen1 Song-Lin Chen1 摘 要： Spermatogenesis within the adult testis is an excellent system for studying stem cell renewal and differentiation, which is under the control of testicular somatic cells. In order to under-standing spermatogenesis in the half-smooth tongue sole -
Establishment, characterization and virus susceptibility of a kidney-derived cell line from southern flounder, Paralichthys lethostigma Jordan & Gilbert
作 者： N Wang1 , X-L Wang1, Z-X Sha1, Y-S Tian1 and S-L Chen1 摘 要： Since the establishment of the first teleost fish cell line in the 1960s (Wolf 1962), more than 100 lines have been established and applied in many aspects of virology, immunology and genetics in freshwater and marine fish (Ferna ´ndez, Yoshimizu, Kimura, Ezura -
Development and characterization of a new marine fish cell line from turbot (Scophthalmus maximus)
作 者： N. Wang • X. L. Wang • Z. X. Sha • Y. S. Tian • S. L. Chen 摘 要： A new marine fish cell line, TK, derived from turbot (Scophthalmus maximus) kidney, was established by the method of trypsin digestion and subcultured for more than 50 passages over a period of 300 days. -
Establishment, characterization of a new cell line from heart of half smooth tongue sole (Cynoglossus semilaevis)
作 者： X. L. Wang • N. Wang • Z. X. Sha • S. L. Chen 摘 要： A new cell line was established from the heart of a culturedmarine fish, half smooth tongue sole (Cynoglossus semilaevis), designated as CSH (Cynog-lossus semilaevis heart cell line). -
Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis)
作 者： SHA Zhenxia1x, REN Guocheng1 2x, WANG Xianli 1 3x, WANG Na1, CHEN Songlin1¤ 摘 要： A new cell line, CSEC, has been successfully established from embryos at gastrula stage of a cultured marine ¯sh, half smooth tongue sole (Cynoglossus semilaevis). CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS (fetal bovine serum), -
Establishment and Characterization of a New Cell Line from the Kidney of Spotted Halibut Verasper variegate
作 者： WANG Xianli ， ，CHEN Songlin ’，SHA Zhenxia ，FAN Tingjun¨，and WANG Na ) 摘 要： In vitro culture of fish cells provides an important tool for studying cellular physiology,molecular biology,func—tional genomlcs，oxicology and transgenlc applications (Hightower and Renfro，1988；Bejar et a1． 2005；Bahich and Borenfreund，1 99 l：Gagn6 and Blaise，2000；Con．ception et a1．，2001：Oh et a1．，2001：Tiago and Laiz6．2008；Ma et a1．，200l：Pombinho et a1．，2004)． -
半滑舌鳎肝脏细胞系的建立与鉴定
作 者： 任国诚 陈松林 沙珍霞 摘 要： 以半滑舌鳎肝脏组织为材料，探索了组织细胞分离培养条件，建立了半滑舌鳎组织细胞培养技术及半滑舌鳎肝脏细胞系。该细胞系的培养基是添加了抗生素、胎牛血清、花鲈血清、成纤维生长因子的 。 形态呈纤维状，在培养基中生长迅速，经 多天的培养，成功传代30多代 -
漠斑牙鲆胚胎细胞系的建立与鉴定
作 者： 任国诚 陈松林 沙珍霞 摘 要： :漠斑牙鲆(Paralichthys lethostigma)是中国近年新开发的重要海水养殖鱼类, 本研究以漠斑牙鲆囊胚期胚胎为材料, 探索了鱼类胚胎细胞分离和培养的条件, 建立了漠斑牙鲆胚胎细胞培养技术。建立的漠斑牙鲆胚胎细胞系(SFEC)至今已经培养了240 多天, 传至80 多代。SFEC 的完全培养基采用添加抗生素、胎牛血清(FBS)、花鲈血清(SPS)、成纤维生长因子(bFGF)的DM EM 。SFEC 形态小而呈圆形、多边形, 在培养基中生长迅速。本实验检测了温度、胎牛血清浓度、成纤维生长因子对SFEC 生长的影响。在24 ～ 30 ℃之间SFEC 生长良好, 但当温度低于18 ℃时细胞生长速度明显减慢。在含15% FBS 的培养基中, SFEC 生长速度明显比在含7.5 % FBS 的培养基中快, 在培养基中添加bFGF 可以使细胞生长速度显著提高。SFEC 的二倍体核型为2n =6st42t 。GFP 报告基因通过脂质体介导的方法转入SFEC 中并成功地获得了表达, 转化率为20 %。[ 中国水产科学, 2007 , 14(4):579 -583] -
Pluripotency and Chimera Competence of an Embryonic Stem Cell Line from the Sea Perch (Lateolabrax japonicus)
作 者： Song-Lin Chen,1 Zhen-Xia Sha,1 Han-Qing Ye,1 Yang Liu,1 Yong-Sheng Tian,1 Yunhan Hong,2 Qi-Sheng Tang1 摘 要： A stable GFP-expressing (GFP+LJES1) cell strain was developed from the LJES1 cells obtained from sea perch (Lateolabrax japonicus,) embryos. GFP+LJES1 cells were induced in vitro by RA to differentiate into a variety of cell types and also had the ability to form embryoid body-like structures in suspension culture. To determine the differentiation potential of LJES1 cells in vivo, GFP+ LJES1 cells were trans-planted into sea perch and zebrafish embryos at mid-blastula stage. -
Development and characterization of cell lines from heart, liver, spleen and head kidney of sea perch Lateolabrax japonicus
作 者： H.-Q. YE*†, S.-L. CHEN*‡§, Z.-X. SHA* AND M.-Y. XU* 摘 要： Five cell lines (LJHK, LJS, LJL, LJH-1 and LJH-2) were established from the head kidney, spleen, liver and heart of sea perch Lateolabrax japonicus. The cell lines LJHK, LJS, LJL, LJH-1 and LJH-2 were subcultured 46, 32, 32, 36 and 34 times in minimum essential medium (MEM) supplemented with foetal bovine serum (FBS), sea perch serum and 10 ng ml 1 basic fibroblast growth factor (bFGF). -
Development and characterization of a continuous embryonic cell line from turbot (Scophthalmus maximus)
作 者： Song-Lin Chena,T, Guo-Cheng Rena,b, Zhen-Xia Shaa, Yunhan Hongc 摘 要： A continuous cell line, TEC (turbot embryonic cell line), was established from embryos at the gastrula stage of a cultured marine fish, turbot (Scophthalmus maximus), and has been cultured for more than 200 days with more than 60 passages. The TEC cells were cultured in DMEM medium supplemented with antibiotics, fetal bovine serum (FBS), sea perch serum (SPS), and basic fibroblast growth factor (bFGF). -
Establishment of a pluripotent embryonic cell line from sea perch (Lateolabrax japonicus) embryos
作 者： Song-Lin Chen *, Zhen-Xia Sha, Han-Qing Ye 摘 要： Pluripotent embryonic stem (ES) cells provide an efficient approach for genome manipulation with many applications in marine biotechnology and development studies. To develop this technology, we have worked to derive fish ES cells for in vitro studies of embryo cell growth and differentiation and for the generation of transgenic fish. -
Derivation of a pluripotent embryonic cell line from red sea bream blastulas
作 者： S.-L. CHEN*†, H.-Q. YE*‡, Z.-X. SHA* AND Y. HONG§ 摘 要： A pluripotent cell line, sea bream embryonic stem-like cells (SBES1), was developed from blastula-stage embryos of the cultured red sea bream, Chrysophrys major. The SBES1 cells were cultivated in Dulbecco’s modified eagles medium (DMEM) medium supplemented with foetal bovine serum, marine fish serum, fish embryo extract, selenium, basic fibroblast growth factor and leukemia inhibitory factor.